Evaluation of genetic variability Rindera using RAPD markers

Abstract

Genetic diversity investigations are critical for understanding how to conserve and manage plant resources in every setting. Six Rindera species are reported in Iran. No detailed Rindera genetic diversity was investigated using Random Amplified Polymorphic DNA (RAPD) experiments. Six plants from Iran's seven provinces were gathered and studied for our scientific purposes. Seventy plant specimens have been gathered in total. Our objectives were as follows: 1) to determine genetic variability among Rindera species, and 2) is there a correlation between the genetic and geographical distance of the species? 3) Populations and taxon genetic structure we revealed that quantitative morphological features varied significantly across plant species. Rindera species were classified into two groups using the unweighted pair group approach with arithmetic mean and principal component analysis. The unbiased expected heterozygosity (UHe) of Rindera Regia was in the 0.18 range. Rindera lanata has important Shannon information (0.30). The lowest value was 0.22 for Rindera Regia. In R. cyclodonta and Rindera media, the observed number of alleles (Na) varied between 0.33 and 0.49. R. cyclodonta and Rindera bungei had Ne values between 1.034- 1.17, indicating an effective number of alleles. Rindera has a comparatively low gene flow (Nm) (0.45). According to the Mantel test, there was a significant correlation (r = 0.33, p=0.0001) between genetic and geographical distances. We identified a substantial level of genetic variation, which demonstrates that the Rindera species can adapt to altering environments because genetic diversity is associated with species adaptability. The current findings indicated the efficacy of RAPD markers and morphometry approaches for studying genetic variation in Rindera species.