Response of wheat genotypes to excess boron estimated by in vitro culture
Author:
Kondic-Spika Ankica1, Glogovac Svetlana1ORCID, Trkulja Dragana1ORCID, Marjanovic-Jeromela Ana1ORCID, Marjanovic Milica2
Affiliation:
1. Institute of Field and Vegetable Crops, Novi Sad, Serbia 2. Gimnazija “Svetozar Marković”, Novi Sad, Serbia
Abstract
The objective of this study was to evaluate boron tolerance of wheat
genotypes using mature embryo culture. The analysis involved 79 recombinant
inbred lines of the International Triticeae Mapping Initiative (ITMI)
population and three Serbian varieties with known boron (B) tolerance
(Pobeda - sensitive S, Balerina-medium tolerant MT, and Nevesinjka -
tolerant T). The evaluation was performed on a modified MS medium to which
15 mM of boric acid was added. The control medium contained no excess B.
Callus fresh weight (CFW) and reduction of fresh callus weight (RFCW) were
determined after one month of cultivation. ANOVA has shown highly
significant effect of genotype, the media, and their interaction to callus
tissue growth and also significant genotypic effect on RFCW. Majority of
genotypes (39) had sensitive reaction to excess boron, twenty-three were
medium tolerant, while four of them were tolerant. The obtained results can
be potentially used for mapping QTLs associated with tolerance to excess B
in wheat breeding program.
Funder
Ministry of Education, Science and Technological Development of the Republic of Serbia
Publisher
National Library of Serbia
Subject
Plant Science,Genetics
Reference54 articles.
1. AĞIL F., M., ÖRGEÇ, F.P., KARAKAŞ, S.K., VERMA, N., ZENCIRCI (2022): In vitro mature embryo culture protocol of einkorn (Triticum monococcum ssp. monococcum) and bread (Triticum aestivum L.) wheatunder boron stress. Plant Cell, Tissue and Organ Cult., 148:293-304. 2. ARIF, M.R., M.T., ISLAM, A.H.K., ROBIN (2019): Salinity stress alters root morphology and root hair traits in Brassica napus. Plants, 8:192. 3. ASIF M.A., M., GARCIA, J., TILBROOK, C., BRIEN, K., DOWLING, B., BERGER, R.K., SCHILLING, L., SHORT, C., TRITTERMANN, M., GILLIHAM, D., FLEURY, S.J., ROY, A.S., PEARSON (2020): Identification of salt tolerance QTL in a wheat RIL mapping population using destructive and non-destructive phenotyping. Funct. Plant Biol., 48: 131-140. 4. BEN-GAL, A., U., SHANI (2002): Yield, transpiration and growth of tomatoes under combined excess boron and salinity stress. Plant Soil, 247: 211-221. 5. BOGACKI, P., D.M., PECK, R.M., NAIR, J.H., KLAUS, H., OLDACH (2013): Genetic analysis of tolerance to Boron toxicity in the legume Medicago truncatula. BMC Plant Biol., 13: 54.
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献
|
|