Affiliation:
1. School of Foreign Language, Chizhou University, Chizhou City, China + School of Humanities, Tongji University, Shanghai City, China
2. School of Foreign Language, Chizhou University, Chizhou City, China
Abstract
Yellow hornpoppy (Glaucium flavum Crantz.) is a herbaceous plant with
gray-green leaves in coastal sands, rocky areas, and heavily eroded soils up
to 500 meters above sea level. Glaucium flavum is native to Northern Africa,
temperate zones in Western Asia and Europe, and is indigenous to Iran. The
plant has been widely recognized for its aporphine-type isoquinoline
alkaloids, which are pharmacologically active. Thus, we conducted a
combination of morphological and molecular data analysis on such species
because of the plant species' relevance. One hundred seven randomly
collected plants from 14 natural populations in 5 provinces were evaluated
using ISSR markers and morphological traits. The evaluation of molecular
variance (AMOVA) demonstrated significant genetic divergence between the
examined populations. It indicated that 25% of overall genetic variability
was related to intra-population variety, whereas 75% was due to
inter-population genetic differentiation. ISSR primers discovered 156 bands,
139 (83 %) of which have been polymorphic, each primer containing an average
of 13 bands. The Polymorphic Bands (PPB) Percentage (ISSR-6) varied from 50%
to 100%. (ISSR-1, ISSR-4, and ISSR-5). The average polymorphic information
content (PIC), Shannon's information indexes (I), and several effective
alleles (Ne) were correspondingly 0.39, 0.26, and 1.2.
Publisher
National Library of Serbia