Sensitive voltammetric detection of yeast RNA based on its interaction with Victoria Blue B

Abstract

Voltammetric studies of the interaction of yeast RNA (y-RNA) with Victoria Blue B (VBB) are described in this paper. Furthermore, a linear sweep voltammetric method for the detection of y-RNA was established. The reaction conditions, such as acidity and amount of buffer solution, the concentration of VBB, the reaction time and temperature, etc., were carefully investigated by second order derivative linear sweep voltammetry. Under the optimal conditions, the reduction peak current of VBB at -0.75V decreased greatly after the addition of y-RNA to the solution without any shift of the reduction peak potential. Based on the decrease of the peak current, a new quantitative method for the determination of y-RNA was developed. The effects of co-existing substances on the determination were carefully investigated and three synthetic samples were determined with satisfactory results. The stoichiometry of the VBB-y-RNA complex was calculated by linear sweep voltammetry and the interaction mechanism is discussed.