Affiliation:
1. School of Medicine, Department of Chemistry, Belgrade
2. Faculty of Chemistry, Belgrade
Abstract
Pseudomonas aeruginosa NCAIM(P), B001380, a propitious bacterial strain
isolated from mineral cutting oil was identified to be chromium tolerant and
a producer of biosurfactant rhamnolipid (RL) with potential application in
heavy metal bioremediation. Culture growth, RL production and Cr(VI) removal
capacity of the strain in the presence of 50 mg L-1 (I) and 100 mg L-1 of
Cr(VI) (II) were studied. Maximum of RL production were found in the
late-stationary phase at 72 h for both Cr(VI)-amended cultures: I (236 mg
L-1) and II (160 mg L-1), as well as the maximum of Cr(VI) removal capacity:
70 % (I) and 57 % (II). The amount of Cr in RL preparation II was 22 mg mg-1
determined by flame atomic absorption spectroscopy (FAAS). Appearance of a
new band at 914 cm-1 in infrared (IR) spectrum of RL (II) indicated a
significant proof for possible coordination of CrO42-ion with RL. The effect
of Cr(VI) on monorhamnolipids (RL1) and dirhamnolipids (RL2) distribution and
its ratio were studied by electrospray ionization mass spectrometry (ESI-MS).
An increase was observed in a RL2/RL1 ratio for II compared to control.
Publisher
National Library of Serbia
Cited by
9 articles.
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