Identification of proteins in fluid collected from nerve regeneration chambers

Abstract

We examined whether there are novel neurotrophic factors (NTFs) in nerve regeneration conditioned fluid (NRCF). Nerve regeneration chamber models were established in the sciatic nerves of 25 New Zealand rabbits, and NRCF was extracted from the chambers l week postoperatively. Proteins in NRCF were separated by native polyacrylamide gel electrophoresis (PAGE), and Western blot and ELISA were used to identify the proteins. A novel NTF was identified in a protein fraction corresponding to 220 kDa.