Affiliation:
1. Institute of Field and Vegetable Crops, Novi Sad, Serbia
2. University of Novi Sad, Institute of Lowland Forestry and Environment, Novi Sad, Serbia
Abstract
Monitoring the health status of narrow-leaved ash tree seedlings (forest
office Morovic, locality Vinicna) in the early spring of 2015, after the
catastrophic floods in May 2014, revealed presence of large dark necrotic
areas on 1-2 year old sprouts. The isola?tion of the fungal pathogen was
done by standard phytopathological protocols. Three repre?sentative isolates
(K41, K42 and K78) were preliminary detected and purified by a single-spore
technique for further morphological, molecular analyses and pathogenicity
testing. Morphological characteristics classified the isolates as Fusarium
tricinctum. Tested isolates on narrow leaved ash sprouts caused reddish
brown elongated necrotic lesions averaged 20.1 mm. Two marker genes,
translation elongation factor 1-alpha (TEF1-?) and internal transcribed
spacer (ITS1), were used in this study. Using the Basic Local Alignment
Search Tool (BLAST) searching engine, nucleotide sequences were compared to
all related sequences. Alignement score resulted in 98.9% identities with F.
tricinctum for isolate K78, while isolates K41 and K42 showed 94.1% and
94.3% identities with F. tricinctum complex respectively. To the best of our
knowledge, this is the first report of F. tricinctum pathogen infection on
flood stressed narrow-leaved ash trees in Serbia.
Funder
Ministry of Education, Science and Technological Development of the Republic of Serbia
Publisher
National Library of Serbia
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