Affiliation:
1. Dr.H.S. Gour Vishwavidyalaya, Department of Microbiology and Biotechnology, Sagar, India
2. Yeungnam University, School of Biotechnology, Department of Food Science and Technology, Gyeongsan, Gyeongbuk, Republic of Korea
Abstract
In the present study, a total of 353 nodule-associated bacteria were isolated
from 220 legume plant samples belonging to Cicer arietinum (85), Glycine max
(74), Vigna radiata (21) and Cajanus cajan (40). A total of 224 bacteria were
identified as fast-growing Rhizobium spp. on the basis of differential
staining (Gram staining and carbol fuchsin staining) and biochemical tests.
All the isolates were tested for indole acetic acid production (IAA),
phosphate solubilization and siderophore production on plate assay. To
examine the effect of volatile organic metabolites (VOM) and water soluble
soil components (WSSC) on nodule bacteria, culture conditions were optimized
by observing the effects of various parameters such as pH, salt content and
temperatures on the growth of bacteria. Selected rhizobia were subjected to
random amplified polymorphic DNA (RAPD) and amplified ribosomal DNA
restriction analysis (ARDRA) analysis to identify their species. On the basis
of RAPD and ARDRA, 10 isolates were identified as Rhizobium meliloti. In this
study, Rhizobium GO4, G16, G20, G77, S43, S81, M07, M37, A15 and A55 were
observed as the best candidates among the tested bacteria and can be further
used as potent bioinoculants.
Publisher
National Library of Serbia
Subject
General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology
Cited by
1 articles.
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