A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain-Reference-Cited by-同舟云学术

A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain

Published:2023 Issue:10 Volume:88 Page:985-997
ISSN:0352-5139
Container-title:Journal of the Serbian Chemical Society
language:en
Short-container-title:JSCS

Author:

Sokarda-Slavic Marinela¹^ORCID,Ralic Vanja²^ORCID,Nastasijevic Branislav³^ORCID,Matijevic Milica²^ORCID,Vujcic Zoran⁴^ORCID,Margetic Aleksandra¹^ORCID

Affiliation:

1. University of Belgrade, Institute of Chemistry, Technology and Metallurgy – National Institute of the Republic of Serbia, Department of Chemistry, Belgrade, Serbia

2. Center for light-based research and technologies, COHERENCE, Department of Atomic Physics, Vinča Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, Belgrade, Serbia

3. Vinča Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, Belgrade, Serbia

4. University of Belgrade, Faculty of Chemistry, Department of Biochemistry, Belgrade, Serbia

Abstract

Poly(?-glutamic acid) (PGA), naturally produced by Bacillus species, is a biodegradable, non-toxic, biocompatible and non-immunogenic negatively charged polymer. Due to its properties, it has found various applications in the food, cosmetic and pharmaceutical industries. In this work, Bacillus subtilis 17B was selected as the best PGA producer among fifty wild-types Bacillus strains tested and characterized as a glutamate-independent producer. The production of PGA by the newly identified strain was optimized and increased tenfold using the Box?Behnken experimental design. The purity of PGA after recovery and purification from the fermentation broth was confirmed by SDS- -PAGE followed by methylene blue staining. PGA was characterized by ESI MS and used for the preparation of a new nanocomposite with TiO2. The synthesis of PGA/TiO2 nanocomposite, its structural analysis, and cytotoxic effect on the cervical cancer cell line (HeLa cell) was investigated to determine the potential anti-cancer usage of this newly prepared material. It is encouraging that PGA/TiO2 nanocomposite showed an increased cytotoxic effect compared to TiO2 alone.

Funder

Ministry of Education, Science and Technological Development of the Republic of Serbia

Publisher

National Library of Serbia

Subject

General Chemistry

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