Affiliation:
1. Institute for Biological Research, Belgrade
Abstract
Impatiens walleriana L. shoots were inoculated with Agrobacterium rhizogenes
A4M70GUS and the effects of genetic transformation on the catalase (CAT),
superoxide dismutase (SOD) and peroxidase (POX) activities in wounded region
of stems and unwounded leaves were evaluated 10, 24, 240 and 720 hours after
inoculation. Following Agrobacterum infection activities of plant
antioxidative enzymes changed in a time-dependent manner indicating that
dynamic processes occurred during plant-Agrobacterium interaction, plant
cell transformation and formation of hairy roots. Appearance of hairy roots
on wound sites of shoots was observed ten days after inoculation with A.
rhizogenes and the root induction frequency was 100%. Among selected hairy
root lines significant differences in growth rate and biomass production were
observed and an average 3-fold increase in biomass production was observed
for the best growing hairy root line compared with the untransformed roots.
PCR analysis showed presence of uidA, rolB, rolC and rolD genes in all
analyzed I. walleriana L. hairy root lines, while amplification fragment of
rolA gene was detected in 83.3% transformed lines. Efficient transformation
protocol for I. walleriana L described in this work offer possibilities to
generate hairy root cultures for in vitro propagation of plant viruses.
Funder
Ministry of Education, Science and Technological Development of the Republic of Serbia
Publisher
National Library of Serbia
Cited by
3 articles.
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