Genetic characterization and molecular mapping of novel chlorophyll deficiency gene in air-cured tobacco (Nicotiana tabacum L.)

Abstract

This study was performed to genetical and morphological investigation of a novel chlorophyll deficiency gene in tobacco leaf. One low chlorophyll content (LCC) variety (Urumieh 2) and high chlorophyll content (HCC) variety (Burley Ree 103) from the Burley type was crossed and the F2 generation was grown on the field. One hundred plants were selected, contained low and high chlorophyll content. These plants were sampled and DNA was extracted. Sixty RAPD primers were tested on parents, LCC and HCC Bulks based on Bulk Segregant Analysis (BSA). Chi-square test confirmed the monogenic segregation. Regresion analysis showed that there was strong relationship between greenness degree and chlorophyll contents. Four primers (OPE17, OPC09, OPB08 and OPR02) showed polymorphism and after the test on 97 samples from the F2 generation two markers were selected (OPB08-1050 and OPC09-1900). That showed 15.9 and 10.8 CM distance from chlorophyll locus respectively.