Effect of steroids on transcription and secretion of Gal-1 by the human trophoblast cell line in vitro

Author:

Cujic Danica1,Bojic-Trbojevic Zanka1,Tosic Natasa2,Pavlovic Sonja2,Vicovac Ljiljana1

Affiliation:

1. Institute for the Application of Nuclear Energy -INEP, Laboratory for Biology of Reproduction, Belgrade

2. Institute of Molecular Genetics and Genetic Engineering, Laboratory for Molecular Hematology, Belgrade

Abstract

Galectin-1 (Gal-1) is a lectin with recently documented pro-invasive function in trophoblasts in vitro, whose regulation is currently insufficiently known. The potential involvement of steroid hormones, synthetic glucocorticoid dexamethasone (DEX), the sex steroid progesterone (PRG) and mifepristone (RU486) in the regulation of Gal-1 in the trophoblast-derived cell line HTR-8/SVneo was investigated. Gal-1 mRNA levels were assessed by real-time PCR. The effect on secretion of Gal-1 into the culture media was followed using the SELDI-TOF protein chip array. We present evidence that DEX and RU486 significantly reduced Gal-1 in the HTR-8/SVneo cell line at the mRNA level. In addition, trophoblast-derived HTR-8/SVneo cells were shown to secrete detectable Gal-1 protein, which was only slightly increased by PRG. The potential clinical relevance of these findings remains to be determined.

Funder

Ministry of Education, Science and Technological Development of the Republic of Serbia

Publisher

National Library of Serbia

Subject

General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology

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