Affiliation:
1. All-Russian Research Institute of Plant Protection
Abstract
Potato spindle tuber viroid (PSTVd) is a naked, circular, single-stranded RNA (356–363 nucleotides in length) which lacks any protein-coding sequences. It is an economically important pathogen and is classified as a high-risk plant quarantine disease. Moreover, it is known that PSTVd is mechanically transmitted by vegetative plant propagation through infected pollen, and by aphids. The aim of this study is to determine the possibility of viroid transmission by potato pathogen Phytophthora infestans (Mont.) de Bary. PSTVd-infected (strain VP87) potato cultivars Gala, Colomba, and Riviera were inoculated with P. infestans isolate PiVZR18, and in 7 days, after the appearance of symptoms, re-isolation of P. infestans on rye agar was conducted. RT-PCR diagnostics of PSTVd in a mixture of mycelia and sporangia were positive after 14 days of cultivation on rye agar. The PSTVd-infected P. infestans isolate PiVZR18v+ was used to inoculate the healthy, viroid-free plants of potato cv. Gala and tomato cv. Zagadka. After 60 days, an amplification fragment of PSTVd was detected in the tissues of one plant of tomato cv. Zagadka by RT-PCR with the primer set P3/P4, indicating successful transmission of PSTVd by P. infestans isolate PiVZR18v+. This result was confirmed by sequencing of the RT-PCR amplicon with primers P3/P4. The partial sequence of this amplicon was identical (99.5 %) to PSTVd strain VP87. RT-PCR showed the possibility of viroid stability in a pure culture of P. infestans isolate PiVZR18v+ after three consecutive passages on rye agar. PSTVd was not detected after the eighth passage on rye agar in P. infestans subculture. These results are initial evidence of potato viroid PSTVd being bidirectionally transferred between P. infestans and host plants.
Publisher
Institute of Cytology and Genetics, SB RAS
Subject
General Biochemistry, Genetics and Molecular Biology,General Agricultural and Biological Sciences
Reference49 articles.
1. Andika I.B., Weia Sh., Cao Ch., Salaipetha L., Kondo H., Suna L. Phytopathogenic fungus hosts a plant virus: a naturally occurring cross-kingdom viral infection. Proc. Natl. Acad. Sci. USA. 2017;114(46):12267-12272. DOI 10.1073/pnas.1714916114.
2. Behjatnia A., Dry I., Krake L., Condé B.D., Connelly M.I., Randles J., Rezaian M.A. New potato spindle tuber viroid and tomato leaf curl geminivirus strains from a wild Solanum sp. Phytopathology. 1996;86:880-886. DOI 10.1094/Phyto-86-880.
3. Bhat Al.I., Rao G.P. Characterization of Plant Viruses. Methods and Protocols. Humana, New York: Springer Science+Business Media, 2020. DOI 10.1007/978-1-0716-0334-5_1.
4. Black W., Mastenbroek C., Mills W.R., Peterson L.C. A proposal for an international nomenclature of races of Phytophthora infestans and of genes controlling immunity in Solanum demissum derivatives. Euphytica. 1953;2:173-178. DOI 10.1007/BF00053724.
5. Brown D.J.F., Ploeg A.T., Robinson D.J. A review of reported associations between Trichodorus and Paratrichodorus species (Nematoda : Trichodoridae) and tobraviruses with a description of laboratory methods for examining virus transmission by trichodorids. Rev. Nématol. 1989;12(3):235-241.
Cited by
8 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献