Study of the immunotoxic properties of pegylated hyaluronidase-Reference-Cited by-同舟云学术

Study of the immunotoxic properties of pegylated hyaluronidase

Published:2023-10-30 Issue:5 Volume:43 Page:118-126
ISSN:2410-2520
Container-title:Сибирский научный медицинский журнал
language:
Short-container-title:Sibirskij nauchnyj medicinskij zhurnal

Author:

Ershov K. I.¹^ORCID,Shvetsova A. M.²^ORCID,Sherstoboev E. Yu.³^ORCID,Churin A. A.³,Zhdanov V. V.³,Korolev M. A.²^ORCID,Madonov P. G.¹^ORCID

Affiliation:

1. Novosibirsk State Medical University of Minzdrav of Russia; Research Institute of Clinical and Experimental Lymphology – Branch of the Federal Research Center Institute of Cytology and Genetics SB RAS

2. Research Institute of Clinical and Experimental Lymphology – Branch of the Federal Research Center Institute of Cytology and Genetics SB RAS

3. Research Institute of Pharmacology and Regenerative Medicine E.D. Goldberg Tomsk National Research Medical Center of the Russian Academy of Sciences

Abstract

The development of safe drugs occupies a special place in the pharmaceutical industry. One of the main tasks of its preclinical phase is to evaluate possible toxic effects of the developed drug on the body and on various systems, including the immune system. The aim of our work was to study immunotoxic properties of pegylated hyaluronidase (PEG- HYAL). Material and methods. Mice F1(CBA/C57Bl/6) were divided into subgroups which were intragastric and intraperitoneally administered with PEG-HYAL in different dosages (50, 500, 1250, 2500 and 5000 U/kg). The number of antibody-producing cells in the spleen, the mass and cellularity of central and peripheral immune organs, phagocytic activity of peritoneal macrophages and neutrophils, delayed hypersensitivity reaction (DHR), level of hemagglutinin to sheep erythrocytes, spontaneous and mitogen-induced splenocyte proliferation were determined. Results. PEG-HYAL did not induce DHR, did not suppress phagocyte activity of peritoneal macrophages, at a dose of 50 ED/kg did not significantly affect the hemagglutinin content to erythrocytes, but at a dose of 500 ED/kg did statistically significantly reduce the titer of specific antibodies. When experimental animals were exposed to PEG-HYAL at doses of 50 and 500 U/kg, spontaneous and mitogen-induced proliferation of splenocytes decreased. Conclusions. The PEG-HYAL trial produced results that can be used to substantiate the administration of the PEG-HYAL-based medication.

Publisher

Institute of Cytology and Genetics, SB RAS

Subject

General Biochemistry, Genetics and Molecular Biology

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