Efficacy of a formalin-inactivated vaccine against Lactococcus garvieae infection in golden pompano Trachinotus ovatus

Author:

Lv Jing1,Yang Lindi1,Li Yanping1,Yang Shiping12,Cai Shuanghu12,Jian Jichang12,Huang Yucong12

Affiliation:

1. Fisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang, China

2. Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen, China

Abstract

Lactococcus garvieae is one of the primary pathogens causing the severe disease to golden pompano (Trachinotus ovatus). Vaccination is the most effective measure of preventing the occurrence and spread of infectious diseases. However, no commercially available vaccine protects T. ovatus against L. garvieae infection. In the present study, the formalin-inactivated L. garvieae vaccines, consisting of bacterin alone (aqueous vaccine) and combined with oil adjuvant Montanide™ ISA 763 A VG (vaccine+763A), were prepared and administered to T. ovatus by intraperitoneal injection. The protective efficacy was evaluated by testing the relative percent survival (RPS), serum enzyme activity, and expression levels of immune-related genes in the tissues of T. ovatus after immunization. The results indicated that the relative percentage survival (RPS) values of aqueous vaccine and vaccine+763A were 80% and 90.8% at 8 weeks post-vaccination. The level of antibody titer, lysozyme activities, superoxide dismutase, and catalase in the two vaccinated fish groups increased significantly compared to that in the control group. Moreover, the expression levels of immune-related genes IgM, TNF-α, IL-8, MHCI-α, MHCII, CD4, CD8α, and IL-1β in all the tested tissues from T. ovatus were significantly enhanced after inoculation with aqueous vaccine and vaccine+763A. These findings demonstrated that the developed vaccine combined with oil adjuvant ISA 763 A VG improved immune responses and provided good protective efficacy against L. garvieae infection in the golden pompano culture.

Publisher

SAABRON PRESS

Subject

Agronomy and Crop Science,Aquatic Science

Reference29 articles.

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