Efficiency of DNA Extractions Methods from Pigeons and Cockatiels Feathers

Abstract

This study aimed to compare different DNA extraction methods to achieve higher amounts and purity levels from molted feathers of pigeons (Columba livia f. domestica) and cockatiels (Nymphicus hollandicus). We evaluated 226 animals consisting of 202 pigeons and 24 cockatiels for these purposes. We performed three commercially available DNA extraction kits to isolate DNA from the feather samples. These kits were compared regarding DNA yield and quality depending on the different applications made during the isolation. DNA concentration (ng/mL) and absorbance ratio (260/280) were measured using a Nanodrop spectrophotometer. Kruskal-Wallis test with the Dunn’s post hoc comparison was performed for the statistical comparisons. The mean DNA concentration was the highest in isolation with the kit C. Among three commercial kits, statistically significant differences were observed concerning nucleic acid concentration (ng/µL) (P