PRODUCTION OF GLYCOSAMINOGLYCAN (GAG) FROM CHICKEN STERNUM BY ENZYMATIC HYDROLYSIS AND OPTIMIZATION OF PROCESS PARAMETERS-Reference-Cited by-同舟云学术

PRODUCTION OF GLYCOSAMINOGLYCAN (GAG) FROM CHICKEN STERNUM BY ENZYMATIC HYDROLYSIS AND OPTIMIZATION OF PROCESS PARAMETERS

Published:2022-08-15 Issue: Volume: Page:650-662
ISSN:1300-3070
Container-title:GIDA / THE JOURNAL OF FOOD
language:tr
Short-container-title:GIDA

Author:

KARAMAN Çise¹,KUMCUOĞLU Seher²,KAYA BAYRAM Sibel,TAVMAN Şebnem²

Affiliation:

1. EGE ÜNİVERSİTESİ, FEN BİLİMLERİ ENSTİTÜSÜ, GIDA TEKNOLOJİSİ (YL) (TEZLİ)

2. EGE ÜNİVERSİTESİ, MÜHENDİSLİK FAKÜLTESİ, GIDA MÜHENDİSLİĞİ BÖLÜMÜ

Abstract

In this study, sulfated glycosaminoglycan (GAG) extraction was performed from chicken sternum by enzymatic hydrolysis method and optimum enzymatic hydrolysis process conditions were determined. Response Surface Method was used for the optimization of the process conditions. In order to determine the effects of the selected four parameters as enzyme amount, pH value, extraction temperature and time on GAG yield, a trial design created with Central Composite Design was applied. It is aimed to reach the highest GAG efficiency in the shortest time and by using the least amount of enzyme. The most suitable process conditions for the extraction process were found to be 0.6% enzyme amount, 6.99 pH value, 62.96°C extraction temperature and 10.79 hours extraction time. Under these conditions, the extraction yield value was predicted to be 26.07% and it was confirmed experimentally.

Publisher

Association of Food Technology, Turkey

Subject

General Medicine

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