Ultra-performance liquid chromatography determination of related compounds of molindone in drug substances-Reference-Cited by-同舟云学术

Ultra-performance liquid chromatography determination of related compounds of molindone in drug substances

Published:2022-06-30 Issue:2 Volume:13 Page:180-185
ISSN:2153-2257
Container-title:European Journal of Chemistry
language:en
Short-container-title:Eur J Chem

Author:

Nagarajan Balaji¹^ORCID,Manoharan Gunasekar¹^ORCID,Shanmugam Ganapathy Narayanan¹^ORCID,Palaniyappan Nataraj²^ORCID,Yarragunta Abhinav³^ORCID

Affiliation:

1. New Jersey Bioscience Centre, 675 US Highway 1, North Brunswick, New Jersey, 08902, United States

2. Shri Jagdishprasad Jhabarmal Tibrewala University, Rajasthan, 333001, India

3. Stanton College Preparatory School is a preparatory high school in Jacksonville, Florida, United States

Abstract

Effective chromatographic separation was achieved on a phenyl-hexyl stationary phase (50×2.1 mm, 1.9 micron particles) with the economical and straightforward mobile phase combination delivered in isocratic mode at a flow rate of 0.6 mL/min at 254 nm using a ultra-performance liquid chromatography (UPLC) system. In the developed method, the resolution between molindone and its related compounds was more significant than 2.0. Regression analysis shows an r2 value (correlation coefficient) greater than 0.999 for molindone and its associated compounds. This method could detect related compounds of molindone at a level below 0.009% with respect to a test concentration of 500 µg/mL for a 2.0 µL injection volume. The method has shown good, consistent recoveries for related compounds (90-110%). The test solution was found to be stable in the diluent for 48 hours. The drug was subjected to stress conditions. The mass balance was found to be close to 99.3%.

Publisher

European Journal of Chemistry

Subject

General Medicine

Link

https://eurjchem.com/index.php/eurjchem/article/viewFile/2252/pdf_2252

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