INFLUENCE OF NANOPARTICLES OF HIGHLY DISPERSED SILICA ON MORPHOLOGY AND INTRACYTOPLASMIC LOCALIZATION OF LIPID DROPLETS IN PORCINE OOCYTES

Author:

Novichkova D. A.,Kuzmina T. I.,Shcherbak O. V.,Galagan N. P.,Epishko O. A.

Abstract

Based on the visualization by the fluorescent probe (Nile red) of intracellular lipids in porcine oocytes that have finished growth phase in vivo or in vitro morphology and distribution of lipid drops in oocytes before and after cultivation with nanoparticles of highly dispersed silica (0.001% of HDS) have been characterized. In the cultivation of oocytes with HDS the level of oocytes that have finished growth phase in vitro with lipid droplets in the form of granules and diffuse type of distribution increases in comparison with the above-indicated markers in the oocytes of the other studied groups. The results of the experiments make it possible to interpret the obtained data on the form of lipids in the form of granules, as a form that determines the high potencies of oocytes for further development and assume that the transformation of granules into clusters during cultivation is considered as a predictor of subsequent destructive changes in the oocyte.

Publisher

Publishing House of National Academy Agrarian Sciences of Ukraine

Reference16 articles.

1. Kuzmina T.I. Informazionnie embriotechnologii v reprodukzii jivotnih: ot fundamentalbЂ™nih issledovanii k praktike [Innovative embryotechnology in the reproduction of animals: from basic researches to practice] / T.I. Kuzmina, X. Torner, H. Alm. // Dostizheniya naukiitekhniki APK [Scientific and technological AIC]. - 2010. - b„–4. - P. 66-67.

2. Kuzmina T. I. Modelirovanie system dozrevanya oozitov svinei in vitro [Modelling of maturation systems for porcine oocyte in vitro]/ T.I. Kuzmina, D.A. Novichkova, N.A.Volkova //SelbЂ™skokhozyaistvennaya Biologiya [Agricultural Biology]. - 2013. - b„–2.-P. 52-57.

3. Prates E. G. A Role of Lipid Metabolism during Cumulus-Oocyte Complex Maturation: Impact of Lipid Modulators to Improve Embryo Production/ E. G. Prates, J. T. Nunes, R.M. Pereira// Mediators of Inn¬‚ammation. bЂ“ 2014. - V. 2014. - P. 11.

4. Prates E. G. Fat area and lipid droplet morphology of porcine oocytes during in vitro maturation with trans-10, cis-12 conjugated linoleic acid and forskolin / E. G. Prates, C. C. Marques, M. C. Baptista [et al.] // Animal. - 2013. - V. 7. - P. 602-609.

5. MilakoviD‡ I. Identification of the lipid droplets of immature porcine oocytes during the different stage of folliculogenesis / I. MilakoviD‡, E . HanulGЎkovGЎ, M. JeEЎeta, D. KnitlovGЎ, K. HanzalovGЎ, R. HorskGЅ, L. MGЎchal // Proceedings of International Ph.D. Students Conference. - 2008. - P. 973-980.

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3