NON-STEROIDAL ANTI-INFLAMMATORY DRUGS "CELEXIB" AND "DIBUTALIASTIN" EFFICACY IN INDUCED INFLAMMATION IN RATS

Abstract

Non-steroid Anti-Inflammatory Drugs (NSAIDs) are the most widely used analgesics in veterinary medicine. Induced inflammation in laboratory animals is one of the most common methods to determine the anti-inflammatory and analgesic properties of new drugs. We studied the anti-inflammatory properties of the drugs "Celexib", with the celecoxib (100 mg in 1 ml) active pharmaceutical ingredient and "Dibutalastin” ointment (1 g of the drug contains 61.0 mg of methyl salicylate). The studies resulted in the following, after supplantation of 1% carrageenan solution into laboratory rats, animals of the control and experimental groups developed an acute inflammatory process (swelling, increased volume of the paw, hyperemia, and tenderness). The most significant changes were observed in rats of all groups three hours after carrageenan administration. It was found that rats of the control group that did not receive anti-inflammatory drugs had the volume of the paw increased on the 4th and 6th hours of the experiment. The animals of experimental E1 and E2 groups, after 4 hours showed decreased intensity of the inflammatory process under the influence of "Celecoxib" and "Diclofenac” anti- inflammation drugs. The volume of the paw was 25.6, 8.2, and 14.3% less in the E1 , and E2 groups of animals, compared to the period before induction of inflammatio. By the 6th hour of the study, the animals of group E1 had virtually no visible clinical signs of the inflammatory process caused by carrageenan, which confirms the anti-inflammation effectiveness of the “Celecoxib” drug. It was found that inflammation induced by allyl isothiocyanate and formalin in rats, the investigated drug "Dibutalastin" ointment also showed peculiar analgesic properties. It was observed that its analgesic and anti-inflammatory effects were like the reference drug "Dolaren-gel".