Author:
Keller Katharina,Wiegand Thomas,Cadalbert Riccardo,Meier Beat H.,Böckmann Anja,Jeschke Gunnar,Yulikov Maxim
Abstract
The substitution of Mg2+ by Mn2+ in the bacterial DnaB helicase from Helicobacter pylori, an ATP:Mg2+-fuelled protein engine, allows electron paramagnetic resonance (EPR) spectroscopy to be performed on this system. EPR experiments make it possible
to monitor nucleotide binding and to estimate the fraction of bound Mn2+ through relaxation measurements. Furthermore, by measuring spin–spin distances we probe the geometry within such multimeric assemblies using ultra-wideband double electron-electron resonance (DEER) and
relaxation induced dipolar modulation enhancement (RIDME). The extraction of distance distributions from RIDME experiments on high-spin paramagnetic centres is influenced by the presence of dipolar frequency overtones. We show herein that we can correct for these overtones by using a modified
kernel function in Tikhonov regularization analysis routines, and that the overtone coefficients for Mn2+ in the DnaB helicase are practically the same as in the previously studied Mn2+–Mn2+ model compounds.
Subject
General Medicine,General Chemistry
Cited by
4 articles.
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