Изучение разнообразия ассоциаций фагов гомологичных молочнокислым бактериям

Abstract

Information on the ecology of the "phage-bacterial host" system is given. The interaction of cultures of lactococci from the collection of the Siberian Research Institute of Cheese with associations of phages was investigated. Infection of sensitive cells with bacteriophage led to cell lysis, which was manifested by negative colonies on the lawn of a lactococcal culture. Characteristics of the biological properties of bacteriophages in the composition of phage associations are given (lytic activity, spectrum of lytic activity, specificity of action). Differentiation of species lysotypes of bacteriophages of mesophilic lactococci, selected at a single cheese enterprise in the Altai Territory was noted. The index of the lytic activity of the investigated phage associations ranged from 0.19 (sample of Lactococcus lactis subsp. Lactis biovar diacetylactis) to 0.24 (sample of Lactococcus lactis subsp. Lactis) from studied strains. The value of the lyotype updated coefficient for the entire array of data varied from 0.25 to 1.00. It is concluded that the specificity of cheese biotechnology together with the evolutionary and genetic determinants of the microworld predetermine a constant high risk of cell phagolysis of starter cultures. Experimental studies confirmed the need to consider the phagocyte of lactic acid bacteria in biotechnology.