Molecular characterization and phylogenetic analysis of pseudorabies virus isolated from pigs in Ukraine-Reference-Cited by-同舟云学术

Molecular characterization and phylogenetic analysis of pseudorabies virus isolated from pigs in Ukraine

Published:2023-03-23 Issue:2 Volume:14 Page:180-185
ISSN:2520-2588
Container-title:Regulatory Mechanisms in Biosystems
language:
Short-container-title:Regul. Mech. Biosyst.

Author:

Ukhovskyi V. V.,Romanov O. M.,Chechet O. M.,Sytiuk M. P.,Korniienko L. Y.,Tsarenko T. M.,Radzykhovskyi M. L.,Gerilovych A. P.

Abstract

The article presents the results of a molecular genetic study of two isolates of the Pseudorabies virus that were isolated from pigs in Ukraine. Bioinformatic analysis of the gE gene fragment of Aujeszky's disease virus (Pseudorabies virus) isolates was carried out in order to determine the phylogenetic relationships and homology of nucleotide sequences. Fragments of the Aujeszky disease virus genome corresponding to the C-terminal region of the gE gene were selected for sequencing and further analysis. As a result of the conducted studies, it was demonstrated that the nucleotide sequences of the analyzed samples differ from each other by the presence of ACG insert in the tandem repeats region. Comparison of the studied sequences with the sequences of strains/isolates of the Aujeszky's disease virus found in Europe and Asia, presented in the GenBank database, indicates that such an insert is characteristic for the Min-A and HNJZ strains (position 1487 in the gE gene) isolated in Asia. Analysis of the homology of nucleotide sequences showed that the sequence of the gE gene fragment of sample No. 1 is 100% identical to the sequences of strains 89V87 and 00V72 isolated in Belgium. The homology of the nucleotide sequence of the gE gene fragment of sample No. 3 with strains 89V87 and 00V72 was 99.13%. In order to clarify the analyzed samples belonging to a particular genogroup (genetic cluster), a phylogenetic dendrogram was constructed. This demonstrates the phylogenetic relationships between strains/isolates of the Aujeszky's disease virus. It was found that the analyzed samples belong to the genetic cluster uniting European strains/isolates, and the studied isolates are most genetically close to strains 89V87 and 00V72.

Publisher

Oles Honchar Dnipropetrovsk National University

Subject

Toxicology,Pharmacology,Microbiology,Physiology,Cell Biology,Biophysics,Biochemistry,Biochemistry, Genetics and Molecular Biology (miscellaneous)

Reference41 articles.

1. Abbate, J. M., Giannetto, A., Iaria, C., Riolo, K., Marruchella, G., Hattab, J., Calabrò, P., & Lanteri, G. (2021). First isolation and molecular characterization of pseudorabies virus in a hunting dog in Sicily (Southern Italy). Veterinary Sciences, 8(12), 296.

2. Ai, J. W., Weng, S. S., Cheng, Q., Cui, P., Li, Y. J., Wu, H. L., Zhu, Y. M., Xu, B., & Zhang, W. H. (2018). Human endophthalmitis caused by pseudorabies virus infection, China, 2017. Emerging Infectious Diseases, 24(6), 1087–1090.

3. Bo, Z., & Li, X. (2022). A review of pseudorabies virus variants: Genomics, vaccination, transmission, and zoonotic potential. Viruses, 14(5), 1003.

4. Bo, Z., Miao, Y., Xi, R., Gao, X., Miao, D., Chen, H., Jung, Y. S., Qian, Y., & Dai, J. (2021). Emergence of a novel pathogenic recombinant virus from Bartha vaccine and variant pseudorabies virus in China. Transboundary and Emerging Diseases, 68(3), 1454–1464.

5. Carr, A. N., Milleson, M. P., Hernández, F. A., Merrill, H. R., Avery, M. L., & Wisely, S. M. (2018). Wildlife management practices associated with pathogen exposure in non-native wild pigs in Florida, U.S. Viruses, 11(1), 14.