Antibacterial effect of "Ecocide C" disinfectant against mycobacteria

Abstract

We presented the results of experimental studies on the bactericidal properties of the disinfectant "Ecocide С" (produced by "KRKA", Slovenia) with respect to various types of mycobacteria. The objects of research were 15 cultures of various species of mycobacteria from the collection of pathogens of infectious diseases of animals of the National Scientific Center "Institute of Experimental and Clinical Veterinary Medicine" (Kharkov). The disinfectant "Ecocide C" had bactericidal properties with respect to various species of mycobacteria: M. avium, M. bovis, M. kansasii, M. gordonae, M. scrofulaceum, M. intracellulare, M. terrae, M. triviale, M. xenopi, M. diernhoferi, M. flavescens, M. fortuitum, M. phlei, M. smegmatis, M. thamnopheos. When exposed to bactericidal concentrations, the disinfectant "Ecocide C" caused destruction of the cell wall and cytoplasmic membrane of mycobacteria, destruction of the granular component of the cytoplasm with the formation of osmiophilic fine granular inclusions, and electron-transparent vacuoles. When we applied the disinfectant "Ecocide C" on mycobacterial cells it caused the irreversible changes in the structural elements of mycobacteria and finally the death of microorganisms. Disinfectant "Ecocide C" shown bactericidal properties against atypical mycobacteria and pathogens of tuberculosis of farm animals and poultry at a concentration of 5.0 % with exposure to the action of 24 hours and can be applied in a common complex of antituberculous activities.