Evaluation of antimicrobial properties of polymer nanocomposites for medical application

Author:

Hopta O. V.,Mishyna M. M.,Syrova A. O.,Makarov V. O.,Avramenko V. L.,Мishurov D. O.

Abstract

The paper is devoted to the investigation of antimicrobial activity of polymer nanocomposites of both low-density polyethylene and nonwoven polymeric material (a mixture of woven and polyester fibers) that had been impregnated by Cu nanoparticles. The microorganisms were grown according to generally accepted microbiological rules and on media recommended for each bacteria family. Formation of biofilms of microorganisms was studied on the surface of microtiter plates for enzyme-linked immunosorbent assay. After incubation of microtiter plates, the culture medium was removed from the wells. The wells were rinsed five times with sterile distilled water. The plates were air dried for 45 minutes and each well was stained with 1% crystal violet solution in water for 45 min. After staining, the plates were washed with sterile distilled water five times. The quantitative analysis of biofilm production was done by adding 95% ethanol for discoloration of the wells. The research shows the antibacterial activity of Cu nanoparticles on planktonic forms of the investigated microorganisms, which prevented the formation of dense biofilms. With the use of low-density polyethylene impregnated by Cu the ability to form biofilms by planktonic cells of the referent strains of microorganisms was detected to decrease by 1.7 (Escherichia coli), 12.3 (Klebsiella pneumonia) times in the studied strains and with the use of nonwoven polymeric material treated by Cu nanoparticles, the ability to form biofilms decreased by 1.8 (Escherichia coli) – 21.8 (Klebsiella pneumonia) times in the studied strains. In subjecting the formed daily biofilms of referent strains of microorganisms to Cu nanoparticles, the destruction of biofilms of the studied strains of microorganism was observed as well as violation of the integrity of the biofilm monolayer and decrease of density index in comparison with control values. As a result, the obtained polymer nanocomposites can be recommended for preventive use in the fight against nosocomial infections. The practical relevance of this study lies in the possibility of reducing the incidence of purulent-inflammatory diseases and mycoses and, accordingly, reduction of the costs of treating these diseases.

Publisher

Oles Honchar Dnipropetrovsk National University

Subject

General Engineering

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3