Affiliation:
1. Russian State University named after A.N. Kosygin (Technologies. Design. Art); Moscow Regional Research and Clinical Institute (MONIKI)
2. JSC "Production Association "Ural Optical and Mechanical Plant named after Mr. E.S. Yalamov" (UOMZ)
3. Moscow Regional Research and Clinical Institute (MONIKI)
4. Mineralnye Vody District Hospital
Abstract
The paper presents a review of current techniques of the interference microscopy of living functioning cells that allow for assessment of the real-time changes of subcellular microstructures with 2D and 3D reconstructions of the images and multifactorial data analysis. It has been shown that new diagnostic information can be obtained within the research methodology of the so-called interactive dialogue with the cell to identify changes in the morphofunctional state of living cells through registration of their realtime response to environmental fluctuations. We describe physical basis of laser interferometry, problems and specifics of interpretation of cell phase pictures. As an illustration to the practical use of the technique in the clinic we present the results of studies on morphofunctional state of peripheral blood platelets in patients with oral malignancies (OM) and various stages of the tumor. Analysis of circulating platelets with a realtime technique of laser interferometry allowed for identification of compensated activation of the platelet compartment of hemostasis in these patients, as well as an association between the severity of cancer and changes of dimensional cell parameters (an increase of the mean diameter and area of circulating platelets by 23.4, 26.8 and 30.3%, and by 30.2, 32.5 and 38.1% in the patients with OM grades II, III and IV, respectively (p < 0,05 for all comparisons)). The necessary condition for a wider implementation of new diagnostic technologies is a critical evaluation of the efficacy of their use in routine practice, identification of their advantages, disadvantages and differences between current and proposed diagnostic platforms.
Publisher
Moscow Regional Research and Clinical Institute (MONIKI)
Reference32 articles.
1. Shlyakhto EV, Konradi SA, Galagudza MM. Translational medicine: yesterday, today, tomorrow. Vestnik Roszdravnadzora. 2016;(1): 47–51. Russian.
2. Waf A, Mirnezami R. Translational-omics: Future potential and current challenges in precision medicine. Methods. 2018;151:3–11. doi: 10.1016/j.ymeth.2018.05.009.
3. Park Y, Choi W, Yaqoob Z, Dasari R, Badizadegan K, Feld MS. Speckle-feld digital holographic microscopy. Opt Express. 2009;17(15): 12285–92. doi: 10.1364/OE.17.012285.
4. Chernaya VV, Borovitskiy VN. Comparative analysis of modern holographic and interference microscopes. Measuring and Computing Devices in Technological Processes. 2010;(2): 36–43. Russian.
5. Vishnyakov GN, Levin GG, Minaev VL, Tsel'mina IYu. Interference microscopy of subnanometer depth resolution: Experimental study. Optics and Spectroscopy. 2014;116(1):156–60. doi: 10.1134/S0030400X14010226.
Cited by
7 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献