Evaluation of Anticancer Activity and Structural Analysis of Biosynthesized Silver Nanoparticles (AgNPs) from Centella Asiatica

Abstract

Introduction: The synthesis of silver nanoparticles (AgNPs) from an ethanolic extract of Centella asiatica leaf, which exhibits anticancer characteristics, was investigated in this study. Green synthesis, a non-harmful procedure for the environment, aids in the production of nanoparticles from Centella asiatica extract. Centella asiatica has long been regarded to have beneficial properties for neurological function, anti-inflammation, and wound treatment. Objective: FT-IR, GC-MS, and thin-layer chromatography were used to find the phytochemicals present in the extract, and the anti-cancer abilities of the nanoparticles were tested against the PC3 cell line. Methods: Soxhlet extraction is used to extract the bioactive, and then nanoparticles are synthesised. Following that, they are subjected to preliminary phytochemical analysis and thin-layer chromatography. These can assess the presence or absence of phytochemicals. Soon after, GCMS is done to reveal the molecular weight of the bioactive compounds using the charge-to-mass ratio. The structural peaks of different functional groups were identified using FT-IR. Under different concentrations, the silver nanoparticles were portrayed against the PC3 cell line of prostate cancer. Result: The concentration of silver nanoparticles was shown to negatively affect cell viability. The IC50 value was 11.8 µg/ml, showing that the inhibition of growth increased with increasing doses of the AgNPs. Conclusion: Our study highlights the significance of green synthesis of AgNPs from Centella asiatica extract, with bioactive compounds identified using GC-MS showing potential as anticancer agents against PC3 cells, suggesting a promising avenue for cancer therapy.