Silver Nanoparticles Bio-genesis from Colpomenia sinuosa and its in-vivo Anti-tumor Efficacy on DLA Inoculated tumor in albino mice

Abstract

Objective: The anti-tumor activity of biosynthesized silver nanoparticles from marine brown seaweed Colpomenia sinuosa against DLA (Dalton’s lymphoma ascites) induced tumor was investigated. Methods: The biosynthesis of silver nanoparticles from marine macroscopic red seaweed C. sinuosa was synthesized by the green synthesis method and characterized by UV–Vis spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction, Thermogravimetric analysis (TGA), scanning electron microscope (SEM), and transmission electron microscopy (TEM). The complete study was done by purchasing 20 to 25 g of male Swiss albino mice from KMCP College of Pharmacy animal experimental laboratory. The Daltons’ lymphoma ascites cell line induced tumor in albino mice was evaluated for anti-tumor activity with the biogenic silver nanoparticles from marine brown seaweed Colpomenia sinuosa and was estimated for tumor cell count, body heaviness, Life expectancy, haematological and biochemical factors, histologic analysis of liver using H&E and PAS staining. Results: The oral administration of the biosynthesized silver nanoparticles from marine brown seaweed Colpomenia sinuosa at 50 mg per kg body weight albino mice were given daily for 14 days. The haematological and biochemical factors along with bodyweight of the animal, cell count (tumor), and cell volume (packed) were analyzed and compared with Dalton’s lymphoma control group of mice. The treatment control group mice with biosynthesized silver nanoparticles exhibited an increase in haematological factors, a decrease in white blood cells, and normalcy of biochemical factors compared to Dalton’s lymphoma group mice. The reduction in body weight of mice, cell count (tumor), and cell volume (packed) were also observed in treatment group mice with biosynthesized silver nanoparticles as compared to Dalton’s lymphoma group mice. Conclusion: The eco-friendly and green synthesis methodology of biosynthesized silver nanoparticles from Colpomenia sinuosa reversed the haematological, and biochemical factors to near normal range against the DLA control group proving the efficacy of the studies. The improvement in the body weight and the life expectancy of the animals also confirmed the anti-tumor efficacy of the biogenic silver nanoparticles.