Author:
Dr. Gurdeep Singh ,Ritesh Patel ,Mukesh Kr. Singh ,Gaurav Agarwal
Abstract
Objective: The present study aimed to investigate the analgesic and anti-inflammatory activity of the Cnidoscolus phyllacanthus (family Euphorbiaceae) ethanolic leaves extract in the laboratory using in vivo methods to justify its traditional use in the above-mentioned pathological conditions.
Methods: Phytochemical screening was done to find the presence of various secondary metabolites of the plant. In vivo, analgesic activity was performed employing the acetic acid-induced writhing test and tail immersion test on Swiss albino mice at doses of 50, 100 and 200 mg/kg body weight. An anti-inflammatory activity test was done on Wistar rats at three doses (50, 100 and 200 mg/kg body weight) using a carrageenan-induced paw oedema test. Data were analyzed by one-way analysis of variance (ANOVA), and Dunnett's t-test was used as the significance test. P value was considered as the minimum level of significance.
Results: Phytochemical screening revealed the presence of coumarin, flavonoids, monoterpenes, diterpenes and naphthoquinones in the extract. In the acetic acid-induced writhing test, the per cent inhibition of writhing response by the extract was 12.82%, 25.64% and 76.67% at 50, 100 and 200 mg/kg doses, respectively (p < 0.01) and showed an increase in pain threshold in tail immersion method. The extract significantly (p < 0.05 and p < 0.01) inhibited carrageenan-induced inflammatory response in rats in a dose-related manner. Per cent, inhibition in paw oedema was 16.39 %, 29.50 % and 52.45 % at 50, 100 and 200 mg/kg doses, respectively.
Conclusion: The results obtained from the tests indicate that the plant might have one or more secondary metabolite(s) having central and peripheral analgesic and anti-inflammatory activity.
Publisher
BSP Books Private Limited
Subject
Pharmacology (medical),Clinical Biochemistry,General Pharmacology, Toxicology and Pharmaceutics,Pharmacy
Reference20 articles.
1. Oyedapo OA, Adewunmi CO, Iwalewa EO, Makanju VO. Analgesic, antioxidant and anti-inflammatory related activities of 21-hydroxy-2, 41-dimethoxychalcone and 4-hydroxychalcone in mice. Journal of Biological Sciences. 2008; 8(1):131-6
2. Anilkumar M. Ethnomedicinal plants as anti-inflammatory and analgesic agents. Ethnomedicine: A source of complementary therapeutics. 2010:267-93.
3. Farnsworth NR, Akerele O, Bingel AS, Soejarto DD, Guo Z. Medicinal plants in therapy. Bulletin of the world health organization. 1985;63(6):965.
4. Chen Y, Tao S, Zeng F, Xie L, Shen Z. Antinociceptive and anti-inflammatory activities of Schefflera octophylla extracts. Journal of ethnopharmacology. 2015 Aug 2;171:42-50.
5. De Araújo Gomes LM, de Lima-Saraiva SR, de Andrade TM, Silva JC, Diniz TC, Barreto VN, Mendes RL, Quintans-Júnior LJ, de Sousa Quintans JS, de Lima JT, da Silva Almeida JR. Antinociceptive activity of the ethanolic extract from barks and leaves of Cnidoscolus quercifolius (Euphorbiaceae) in mice. Journal of Young Pharmacists. 2014;6(2):64.