Characterization by LC-MS/MS and Antioxidant Activity of Extract and its Different Fractions of Calotropis procera Young Leaves

Abstract

Objective: Plants are extremely useful as a source of medication. Calotropis procera is a plant that is used to treat a variety of illnesses in traditional medical and folklore systems. In this work, bioassay guided liquid-liquid fractionation, phytochemical and molecular spectroscopic analysis and the antioxidant activity of young leaves of Calotropis procera were evaluated. Methods: Bioassay-guided fractionation was carried out on the basis of polarity of solvents and UV, FTIR, LCMS profiling were conducted by using the standard methods. The DPPH scavenging assay was used to determine the antioxidant activity. Results: The extractive value for the ethyl acetate fraction was 9.14 % w/w. The Total phenolic content was highest in the water fraction 83.63±0.0788 GAE/g of dry weight of extract. UV spectroscopy was used to standardize the fractions. The Presence of various functional groups in the extract/fraction was confirmed by FTIR spectroscopy. Based on LCMS, fifty compounds were identified in the hydro-ethanolic extract/fractions. Among all the fractions, the chloroform fraction showed the greatest antioxidant properties (IC50 values=8.03 mg/mL). Conclusion: On the basis of the result of the study it can be concluded that Calotropis procera have the potential to become a promising herb for the treatment of various ailments.