Production, characterization, and applications of a murine monoclonal antibody to dog erythrocyte antigen 1.1
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Published:1992-11-15
Issue:10
Volume:201
Page:1549-1552
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ISSN:0003-1488
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Container-title:Journal of the American Veterinary Medical Association
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language:
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Short-container-title:javma
Author:
Andrews Gordon A.1,
Chavey Patricia S.1,
Smith Joseph E.1
Affiliation:
1. From the Department of Veterinary Pathology and Microbiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506-5605.
Abstract
Summary
A murine IgM monoclonal antibody, which recognizes dog erythrocyte antigen (dea) 1.1, has been produced. The antibody correctly identified canine rbc possessing dea 1.1 in a panel of rbc typed by an independent laboratory. Reactivity of the monoclonal antibody was compared with canine anti-dea 1.1 antiserum with 163 rbc samples from 145 dogs. Results of agglutination tests with the 2 reagents were in agreement for all samples. A card agglutination test that uses the monoclonal antibody with blood is described. A monoclonal antibody-based test should facilitate blood typing for dea 1.1 in clinical practice.
Publisher
American Veterinary Medical Association (AVMA)
Subject
General Veterinary
Cited by
1 articles.
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