Affiliation:
1. Department of Clinical Sciences, College of Biomedical and Veterinary Sciences, Colorado State University, Fort Collins, CO
2. Department of Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH
3. Haima Therapeutics LLC, Cleveland, OH
4. Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH
Abstract
OBJECTIVE
To assess the safety and efficacy of the platelet-like nanoparticle (PLN), and to assess its safety in repeated administration.
ANIMALS
6 purpose-bred dogs.
PROCEDURES
The PLN was administered IV at 3 different doses using a randomized crossover design. Each dog received a full dose of 8 X 1010 particles/10 kg, half dose, and 10 times the dose, with a 14-day washout period between doses. Biochemical, prothrombin time, partial thromboplastin time, and fibrinogen analyses were performed at baseline and 96 hours postinfusion. A CBC, kaolin-activated thromboelastography, platelet function assay closure time, and buccal mucosal bleeding time were performed at baseline and 1, 6, 24, 48, 72, and 96 hours postinfusion.
RESULTS
No significant changes were observed over time in the thromboelastography parameters, closure time, and buccal mucosal bleeding time. After the administration of the half dose, hematocrit levels decreased significantly at 1, 6, 24, 48, and 96 hours, with all values within the reference range. The platelet count was decreased significantly at hours 1, 6, 24, 48, and 72 after administration of the half dose, with values less than the reference range at all hours but hour 72. No significant changes in serum biochemistry, coagulation panel, and fibrinogen were observed for all doses. No adverse events were noted during the first infusion. Three dogs experienced transient sedation and nausea after repeat infusion.
CLINICAL RELEVANCE
The PLN resulted in a dilution of hematocrit and platelets, and did not significantly alter hemostasis negatively. The safety of repeated doses should be investigated further in dogs.
Publisher
American Veterinary Medical Association (AVMA)
Subject
General Veterinary,General Medicine
Reference47 articles.
1. Thrombocytopenia in cats: a retrospective study of 41 cases,1993
2. Epidemiologic survey of thrombocytopenia in dogs: a report on 987 cases,1991
3. Retrospective study of 871 dogs with thrombocytopenia,2009
4. Long-term outcome of primary immune-mediated thrombocytopenia in dogs,2018
5. Treatment and predictors of outcome in dogs with immune-mediated thrombocytopenia,2011
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