Point-of-care rapid immunoassay performed on voided urine, refrigerated up to 24 hours, accurately detects bacteriuria

Author:

Grant David C.1,Keebaugh Audrey E.1,Werre Stephen2

Affiliation:

1. Department of Clinical Sciences, College of Veterinary Medicine, Virginia-Maryland Regional College of Veterinary Medicine, Blacksburg, VA

2. Study Design and Statistics Laboratory, Virginia-Maryland Regional College of Veterinary Medicine, Blacksburg, VA

Abstract

Abstract OBJECTIVE To collect voided urine from dogs with clinical signs of lower UTI and determine the diagnostic performance of a commercially available rapid immunoassay (RIA) immediately after urine collection and after refrigeration at 4 and 24 hours. ANIMALS 40 client-owned dogs. METHODS Aerobic urine culture was performed on urine collected by cystocentesis. Urine samples were collected by voiding, and the RIA performed in triplicate within 30 minutes (time 0) and again in triplicate after 4 and 24 hours of refrigeration. Test precision and agreement between culture results and RIA results at each time point were determined, and factors possibly associated with false results investigated. RESULTS 14 of 40 dogs (35%) had UTI verified by aerobic urine culture, and all had positive RIA. Three dogs had false positive RIA results. Sensitivity, specificity, positive predictive value, and negative predictive value of the RIA were 100, 88%, 82%, and 100%, respectively, and results were not different after 4 and 24 hours of refrigeration. Precision was excellent. CLINICAL RELEVANCE This point-of-care RIA, performed on voided urine refrigerated up to 24 hours, rapidly and accurately identifies bacteriuria in dogs with lower urinary tract clinical signs, inexpensively.

Publisher

American Veterinary Medical Association (AVMA)

Subject

General Veterinary

Reference8 articles.

1. International Society for Companion Animal Infectious Diseases (ISCAID) guidelines for the diagnosis and management of bacterial urinary tract infections in dogs and cats;Weese JS,2019

2. Aerobic bacterial flora of the prepuce, urethra, and vagina of normal adult dogs;Ling GV,1978

3. Diagnostic accuracy of a rapid immunoassay for point-of-care detection of urinary tract infection in dogs;Jacob ME,2016

4. Evaluation of a rapid immunoassay for bacteriuria in dogs;Sutter CM,2023

5. Diagnostic accuracy of a point-of-care test using voided urine samples for detection of bacteriuria in dogs with signs of lower urinary tract disease;Grant DC,2021

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