Abstract
To conserve Alnus nitida species a micropropagation technique was developed using shoot apices/nodal stem segments as starting material for establishment of axenic shoot cultures on cytokinin supplemented MS medium. The established shoots were transformed into multiple shoot cultures on a multiplication medium consisting of MS/B5 medium augmented with a combination of BA/Kn (2.5-10 µM) and IAA/NAA/IBA (2 µM). A combination of BA (5 µM) and NAA (2 µM) in MS medium lead to formation of a maximum 32.2±0.6 shoots/explant after 8 weeks culture period. The sufficiently elongated microshoots were later on rooted on an auxin enriched half strength MS medium. The microplants were demonstrated to show 70% survival upon being transferred to small pots containing vermiculite sand mixture.
Publisher
Bishen Singh Mahendra Pal Singh
Reference21 articles.
1. Barghchi, M. (1988). Micropropagation of Alnus cordata (Loisel.) Loisel. Plant Cell Tissue and organ Culture, 15(3): 233-244.
2. Dar, G.H. and Naqshi, A.R. (2001). Threatened flowering plants of the Kashmir Himalaya-A Checklist. Oriental Sci. 23-53.
3. Enrico, R.J., Ramirez, S.S., Mroginski, L.A. and Wall, L.G. (2005). In vitro plant regeneration of Alnus acuminata HBK. ssp. Acuminata and its root nodulation by Frankia. Plant Cell Tissue and Organ Culture, 80: 343-346.
4. Gamborg, O.L., Miller, R.A. and Ojima, K. (1968). Nutrient requirements of suspension cultures of Soybean root cells. Exp. Cell Res. 50: 151-158.
5. Garton, S., Hosier, M.A., Read, P.E. and Farnham, R.S. (1981). In vitro propagation of Alnus glutinosa Gaertn. Hort Sci., 16(6): 758-759.