Affiliation:
1. Forest Research Institute
Abstract
Genomic DNA extraction from forestry tree species require young leaf samples to obtain high-quality DNA for molecular based study. For some study, leaf samples must be collected from remote areas and are difficult to transport long distances. We developed alternative method of DNA extraction from inner bark of Cedrus deodara. We used 2.5% PVP for removal of phenolic compound and apply high concentration of sodium chloride to removes polysaccharides. Extracted DNA gives positive amplification with PCR using random amplification of polymorphic DNA (RAPD) markers.
Publisher
Bishen Singh Mahendra Pal Singh
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