Rapid in vitro Propagation of Dodonaea viscosa (Sapindaceae) Through Axillary Bud Multiplication and Indirect Organogenesis

Author:

Benniamin A.1,Jothi G.2ORCID,Sundari M.3

Affiliation:

1. Botanical Survey of India, Western Regional Centre

2. Loyola College

3. St. Mary’s College

Abstract

Protocols of axillary bud multiplication were established for Dodonaea viscosa (Sapindaceae). Murashig & Skoog’s medium with 0.2 mg/l BAP with 0.01 Naphthalene acetic acid induced high rate of shoot induction and an average of five shoots per node. Subsequent culture enhanced the number of shoots. Callus initiated from the basal cut end explants differentiated in to more than 10 shoots on MS Medium with 0.4mg/l Benzylaminopurine and 0.02mg/l Indole Butric Acid. Eighty per cent of the rooted shoots survived when transferred to greenhouse and subsequently to the field.

Publisher

Bishen Singh Mahendra Pal Singh

Reference19 articles.

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3. Ashtosh, Vadawale, V., Mehta-Bhatt, P. and Dave A.M. (2004). Rapid In vitro Propagation of Ashwagandha (Withania sominfera) through axillary bud multiplication and Indirect Organogenesis. Phytomorphology 54: 1& 2 pp 59 -64

4. Bhattacharya, S. and Bhattacharya, S. (1997). Rapid multiplication of Jasminum officinale L. by in vitro culture of nodal explants. Plant cell Tissue Organ Cult. 51 : 57-60.

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