Adventitious root formation in shoot cuttings of Himalayan cedar (Cedrus deodara Roxb. ex Lamb.) G. Don under mist chamber conditions

Author:

Tamta Sushma1,Palni L. M.2,Nandi S.3

Affiliation:

1. Kumaun University

2. State Biotechnology Programme, Govt. of Uttarakhand

3. G.B. Pant Institute of Himalayan Environment & Development

Abstract

Cedrus deodara is an important tree species of the Himalayan region and its clonal propagation assumes particular importance for rapid multiplication of desirable biotypes (elite). Cuttings taken from young shoots (current season’s growth) were variously treated with solutions of root inducing chemicals for 24 h, planted individually in polybags which were then placed inside a mist chamber. The experiments were conducted in four different seasons, i.e., winter, summer, monsoon and autumn. The most effective treatment was noticed for commonly used fungicide, bavistin (0.05%), that induced good rooting across seasons. Out of the two auxins tried, namely indole-3-butyric acid (IBA) and µ-naphthalene acetic acid (NAA), lower concentration (0.5mM) of IBA was more effective. Auxins were also treated in combination with phloroglucinol and coumarin, which were tried on their own as well, and the rooting success varied considerably. The monsoon season was found most suitable for taking cuttings. The results of this mist chamber based study were also compared with an earlier study carried out under a polythene tunnel, and the root induction was found similar to favour when treated cuttings were placed under mist chamber conditions.

Publisher

Bishen Singh Mahendra Pal Singh

Reference20 articles.

1. Blazich, F.A. (1988). Chemicals and formulations used to promote adventitious rooting. In: Adventitious Root Formation in Cuttings. (Eds. by T.D. Davis; B.E. Haissig & N. Sankhla), Dioscorides Press, Portland, p.132-149.

2. Davis, T.D.; Haissig, B.E. and Sankhla, N. (1988). Adventitious Root Formation in Cuttings. Dioscorides Press, Portland, pp. 315.

3. Donoho, C.W.; Mitchell, A.E. and Sell, H.N. (1962). Enzymatic destruction of 14C labelled indole acetic acid and naphthalene acetic acid by developing Apple and Peach seeds. Proc. Am. Soc. Hort. Sci., 80: 43-49.

4. Hackett, W.P. (1970). The influence of auxin, catechol, and metabolic tissue extracts on root initiation in aseptically cultured shoot apices of the juvenile and adult forms of Hedera helix. J. Am. Soc. Hort. Sci., 95: 398 402.

5. Hartmann, H.T. and Kester, D.E. (1983). Plant Propagation: Principals and Practices. 4th edition. Prentice Hall, New Jersey, 727 p.

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