Affiliation:
1. Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences;
Siberian State Medical University, Tomsk, Russia
2. Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences
Abstract
Localized and metastatic tumors are known to lead to the formation of circulating tumor cell (CTC ) clusters in the blood. Currently, there is a heightened interest in the study of molecular and biological characteristics of CTC s. Recent studies have shown the presence of different populations of CTC s in the blood of cancer patients. Some cells are cancer stem cells, some tumor cells undergo epithelial-mesenchymal transition (EMT), and most CTC s do not have features of either stem cells or EMT.The aim of the study was to evaluate the five-year metastasis-free survival rate in patients with invasive breast carcinoma, depending on the presence of various populations of circulating tumor cells in the blood before treatment.Material and Methods. A prospective study included 47 patients with newly diagnosed invasive breast cancer (T1–4N0–3M0), who were treated at Cancer Research Institute, Tomsk National Research Medical Center. The patients aged 31 to 69 years. The presence of different populations of CTC s in the blood of patients before treatment was determined by multicolor flow cytometry on the BD FACS Canto system, using different fluorochrome-labeled monoclonal antibodies to EpCam, CD 45, CD 44, CD 24, and N-cadherin. Five-year metastasis-free survival was evaluated by the Kaplan–Meier method. The differences were considered significant at p<0.05.Results. The results obtained demonstrated that the presence of both stem-like and non-stem CTC s showing signs of EMT with Epcam+CD 45-CD 44-CD 24-Ncadherin+, Epcam+CD 45-CD 44+CD 24-Ncadherin+, and Epcam(m)- CD 45-CD 44+CD 24-Ncadherin+ phenotypes in the blood of breast cancer patients before treatment reduced the five-year metastasis-free survival rate (p=0.0016, p=0.017 and p=0.011, respectively).Conclusion. Thus, CTC s in the EMT state are informative for liquid biopsy to assess the risk of hematogenous metastasis and can be considered as targets for selection of personalized chemotherapy.
Publisher
Tomsk Cancer Research Institute
Reference21 articles.
1. Kaprin A.D., Starinskiy V.V., Petrova G.V. Malignant neoplasms in Russia in 2018 (morbidity and mortality). Moscow, 2019. 250 р. (in Russian).
2. Yang M.H., Imrali A., Heeschen C. Circulating cancer stem cells: the importance to select. Chin J Cancer Res. 2015 Oct; 27(5): 437–49. doi: 10.3978/j.issn.1000-9604.2015.04.08.
3. Stoecklein N.H., Fischer J.C., Niederacher D., Terstappen L.W. Challenges for CTC-based liquid biopsies: low CTC frequency and diagnostic leukapheresis as a potential solution. Expert Rev Mol Diagn. 2016; 16(2): 147–164. doi:10.1586/14737159.2016.1123095.
4. Zhe X., Cher M.L., Bonfil R.D. Circulating tumor cells: finding the needle in the haystack. Am J Cancer Res. 2011; 1(6): 740–751.
5. Giordano A., Gao H., Anfossi S., Cohen E., Mego M., Lee B.N., Tin S., De Laurentiis M., Parker C.A., Alvarez R.H., Valero V., Ueno N.T., De Placido S., Mani S.A., Esteva F.J., Cristofanilli M., Reuben J.M. Epithelial-mesenchymal transition and stem cell markers in patients with HER2-positive metastatic breast cancer. Mol Cancer Ther. 2012 Nov; 11(11): 2526–34. doi: 10.1158/1535-7163.MCT-12-0460.
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献