Affiliation:
1. N.P. Bechtereva Institute of the Human Brain of the Russian Academy of Sciences;
I.I. Mechnikov North-Western State Medical University
2. I.P. Pavlov First St. Petersburg State Medical University;
I.I. Mechnikov North-Western State Medical University
3. N.P. Bechtereva Institute of the Human Brain of the Russian Academy of Sciences
4. A.M. Granov Russian Scientifc Center of Radiology and Surgical Technologies of the Ministry of Health of the Russia
5. N.P. Bechtereva Institute of the Human Brain of the Russian Academy of Sciences;
St. Petersburg State University
6. I.I. Mechnikov North-Western State Medical University;
V.M. Bekhterev National Medical Research Center of Neurology and Psychiatry;
I.M. Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences
7. I.I. Mechnikov North-Western State Medical University;
Alexandrovskaya Hospital
Abstract
The management of cerebral glioma (CG) remains challenging. Recently, methods based on the study of the expression levels of miRNAs in blood plasma have proven to be promising. The volume of tumor tissue is known to correlate with increased expression levels of microRNA-21 and -210. MicroRNA-15, -16, -34, -126 and -342 are involved in the regulation of tumor proliferative potential, and microRNA-128 is involved in the regulation of metabolic activity.The aim of the study was to evaluate the extended protocol for assessing the expression of microRNA-15, -16, -21, -34, -126, -128, -210, and -342 in the plasma and saliva of CG patients.Material and Methods. The study group consisted of 24 patients with supratentorial glioma (8 men and 16 women aged 41 to 71 years, mean age: 56 years). The control group consisted of 30 volunteers. MicroRNA expression was studied in plasma and saliva according to the StemLoop-RealTime protocol, using fluorescently labeled samples with small miRNA U6 as a reference gene. Statistical analysis was carried out using nonparametric methods.Results. The lack of CG stabilization and the most probable progression of the tumor with a poor prognosis was related to an increase in microRNA-21 and -210 expression levels and decrease in microRNA-128 expression and at least four of microRNA 15, -16, -34, -126, and -342. Stabilization of CG with a high probability of progression was associated with an increase in microRNA-21 or microRNA210 expression levels, decrease in no more than three of microRNA-15, -16, -34, -126, -342 expression levels as well as decrease in microRNA-128 expression. Stabilization of the tumor with a low probability of progression was associated with a decrease in the expression of microRNA-21 and -210 and in no more than one of miRNA-15, -16, -34, -126, -342 below the reference level, with a simultaneous increase in miRNA-128 expression.Conclusion. The determination of microRNA expression in blood plasma and saliva can be one of the important criteria for assessing the prognosis of CG.
Publisher
Tomsk Cancer Research Institute
Reference26 articles.
1. Preusser M., de Ribaupierre S., Wöhrer A., Erridge S.C., Hegi M., Weller M., Stupp R. Current concepts and management of glioblastoma. Ann Neurol. 2011; 70(1): 9–21. doi: 10.1002/ana.22425.
2. Konovalov A.N., Potapov A.A., Loshakov V.A., Olyushin V.E., Ulitin A.Yu., Kornienko V.N., Pronin I.N., Shishkina L.V., Golanov A.V., Tanyashin S.V., Urakov S.V., Kobyakov G.L. Standards, recommendations and options in the treatment of brain tumors in adults. Problems of Neurosurgery. 2006; 2: 3–11. (in Russian).
3. Karsy M., Arslan E., Moy F. Current Progress on Understanding MicroRNAs in Glioblastoma Multiforme. Genes Cancer. 2012; 3(1): 3–15. doi: 10.1177/1947601912448068.
4. Kuznetsov V.A., Tang Z., Ivshina A.V. Identification of common oncogenic and early developmental pathways in the ovarian carcinomas controlling by distinct prognostically significant microRNA subsets. BMC Genomics. 2017; 18(Suppl 6): 692. doi: 10.1186/s12864-017-4027-5.
5. Barciszewska A.M. MicroRNAs as efficient biomarkers in highgrade gliomas. Folia Neuropathol. 2016; 54(4): 369–74. doi: 10.5114/fn.2016.64812.