Anticoagulant, fibrinogenolytic and anti-platelet aggregation activities of Lablab purpureus (L.) Sweet seed radicle aqueous extract

Abstract

The current study explores the anticoagulant, fibrinogenolytic and anti-platelet aggregation activities of Lablab purpureus (L.) Sweet seed radicle extract (LPRE). It is firstly reported that LPRE has protein at a concentration of 1.5 mg/ml and further evaluated for protein in gel electrophoresis. The proteolytic activity of LPRE was evaluated using casein and gelatin as substrate. LPRE showed a specific activity of 0.35 U. LPRE increased the plasma clotting time significantly showing its anticoagulant property. LPRE hydrolyzed the A?, B? and ? chains of fibrinogen. Furthermore, LPRE significantly inhibited the platelet aggregation induced by agonist adenosine diphosphate (ADP).