Abstract
Methanol extract of Knobweed (Hyptis capitata) root has cytotoxic activity on WiDr cells, but the mechanism of its anticancer action as well as the secondary metabolite contents have not been further explored. This study aimed to extract the roots of H. capitata by a graded maceration, using absolute chloroform and absolute methanol. Methanol extract was used for subsequent assays to trace the mechanism of anti-cancer action. Tests carried out include a doubling time test with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as well as an apoptosis test with Annexin V-PI Flow cytometry method. The secondary metabolite content was identified by the Gas Chromatography-Mass Spectrometry (GC-MS). The results showed that, H. capitata root methanol extract treatment caused morphological changes on WiDr cells. Moreover, the treatment suppressed the cellular proliferation, indicating that it can provide an anti-proliferation effect. Furthermore, the extract treatment at IC50 and 2IC50 caused 65.45% and 97.6% of apoptosis cells respectively. These results proved that H. capitata root methanolic extract has anticancer activity with anti-proliferation properties and the ability to trigger apoptosis on WiDr cells. Phytochemical compounds that might play a role as anti-cancer, among others include chatecol, 9-hexadecanoic acid, hexadecanoid acid-ethyl ester, methyl stearate, ferruginol, retinoic acid, campesterol, stigmasterol and ?-sitosterol.
Publisher
Horizon E-Publishing Group
Subject
Plant Science,Biochemistry, Genetics and Molecular Biology (miscellaneous),Ecology,Ecology, Evolution, Behavior and Systematics
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献