Antioxidant and cardioprotective properties of polyphenolic plant extract of Rhus glabra L.

Abstract

This research looks at the heart-healthy and antioxidant effects of a polyphenol mixture made from Rhus glabra by using a lot of different types of experiments. First, the DPPH assay was used to test the antiradical activity. It showed strong free radical scavenging abilities, with an IC50 value of 18.4 ± 1.4 µL. Mitochondria isolated from rat liver were utilized to examine antioxidative effects on mitochondrial K+ATP ion channels and the mitochondrial permeability transition pore (mPTP). Lipid peroxidation (LPO) was measured via malondialdehyde (MDA) production, indicating that the preparation inhibited LPO and oxidative stress in mitochondrial membranes. Further, the study employed an adrenaline-induced ischemia model to evaluate cardioprotective effects. Treatment with the polyphenol preparation significantly reduced enzyme markers such as ALT, AST, CK, and LDH compared to the ischemic group, highlighting its potential in mitigating ischemic damage. Creatine kinase activity assays indicated improved cellular energy metabolism and biochemical profiling revealed enhancements in atherogenic, cardioprotective, and coronary risk indices. Statistical analyses confirmed the significance of these findings, demonstrating that Rhus glabra exhibits potent antioxidant activity, mitigates oxidative stress, and provides cardioprotective benefits. The study's results suggest that Rhus glabra holds substantial therapeutic potential for enhancing cardiovascular health and combating oxidative stress through its antioxidant properties and ability to improve mitochondrial function and protect against ischemic injury.