The cytotoxic effect of phenol and ethanol on the chondrosarcoma-derived cell line OUMS-27

Author:

Verdegaal S. H. M.1,Corver W. E.2,Hogendoorn P. C. W.2,Taminiau A. H. M.1

Affiliation:

1. Department of Orthopaedics

2. Department of Pathology, Leiden University Medical, Center, P. O. Box 9000, J11-R, 2300 RC, Leiden, The Netherlands.

Abstract

Surgery is considered to be the most effective treatment for cartilaginous tumours. In recent years, a trend has emerged for patients with low-grade tumours to be treated less invasively using curettage followed by various forms of adjuvant therapy. We investigated the potential for phenol to be used as an adjuvant. Using a human chondrosarcoma-derived cartilage-producing cell line OUMS-27 as an in vitro model we studied the cytotoxic effect of phenol and ethanol. Since ethanol is the standard substance used to rinse phenol out of a bone cavity, we included an assessment of ethanol to see whether this was an important secondary factor with respect to cell death. The latter was assessed by flow cytometry. A cytotoxic effect was found for concentrations of phenol of 1.5% and of ethanol of 42.5%. These results may provide a clinical rationale for the use of both phenol and ethanol as adjuvant therapy after intralesional curettage in low-grade central chondrosarcoma and justify further investigation.

Publisher

British Editorial Society of Bone & Joint Surgery

Subject

Orthopedics and Sports Medicine,Surgery

Reference19 articles.

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4. Prognostic factors in chondrosarcoma of bone.A clinicopathologic analysis with emphasis on histologic grading

5. Cartilage-forming tumours of bone and soft tissue and their differential diagnosis

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