Variability of hematological parameters in different species of laboratory mice

Abstract

In scientific research, when conducting experiments, the so-called norms which are used to differentiate the state of health and pathology, are important. At the same time, the requirements for the quality of laboratory animals and their standardization as an object of research are increasing. These are mice of different species that are the main objects used in biomedical research in experiments when analyzing the age-related variability and toxicity of drugs of a chemical nature, modeling the effects of a physical nature, which allow us to obtain objective data. The study of hematological parameters of peripheral blood consisted in determining the clinical parameters of normal blood in laboratory mice (males) of various species and ages, being under standard vivarium conditions, obtained from the “Scientific Collection of Experimental Animals “UNU” (http://www.ckp-rf.ru/usu/ 471933) of the Institute of Biology, Federal Research Centre, Komi Science Centre, Ural Branch, RAS. The content of the main blood parameters was determined in four types of mice: outbred mice (1- and 5-months), CBA/lac (5 months), AF (6–11-months), DBA (16-months) belonging to different age groups of animals (immature, sexually mature and old animals). Relative stability and low variability of hematological parameters were established, especially in linear mice species. The data obtained supplement the understanding of the influence of the age and species of laboratory mice on the nature of the content and variability of individual blood elements. Laboratory animals of different species, lines and ages, bred in standard vivarium conditions, can be recommended when choosing them for conducting experiments and studying patterns in biomedical research.