Determination of phenylephrine hydrochloride and Amoxicillin in binary mixture using Derivative Spectrophotometry methods

Abstract

A simple, precise and economical procedure for the simultaneous estimation of Phenylephrine Hydrochloride (PHE) and Amoxicillin (AMX) in formulation has been developed. The absorbance values of first derivative spectrum 228,258 nm andm 241 nm and second derivative spectrum 238, 277 nm and 226 nm was used for the estimation of PHE and AMX, respectively without mutual interference. This method obeyed beerand#39;s law in the concentration range mixing of 2-150mgL -1 PHE with 0,20,100 mgL -1 AMX and 2-240 mgL -1 AMX with 0,20,100 mgL -1 PHE and the second derivative depends on first derivative of the ratios spectra. The proposed methods are extensively validated. All the described methods can be readily utilized for analysis of pharmaceutical formulations.