Spectrophotometric Method for the Determination of Catechol Amines Drugs in Pharmaceutical Preparations

Abstract

Spectrophotometric methods were developed for the estimation of diazotization of metoclopramide hydrochloride and coupling with catecholamine (dopamine and adrenaline) at the same order. The resulting a violet color that appear to have maximum absorption at λmax 566 nm, and λmax (561) adrenaline, and dopamine, respectively. The optimum conditions of the experiment were individually performed to fi nd high absorbance effi ciency for both drugs. Under the best conditions, law Beer’s lambert was obeyed in the concentration of two drugs range of 1–20 mg/L (R2 = 0.9984), (R2 = 0.9965), and lead to obtain the limit of quantitation (LoQ) (μg mL-1) 0.155 and 0.138, and limit of detection (LoD) (μg mL-1) 0.517 and 0.461, adrenaline, dopamine at the same order. The proposed process was applied and validated to estimate adrenaline and dopamine in several samples of pharmaceutical preparations.