MODIFICATION OF ASPERGILLUS NIGER ATCC 11414 GROWTH FOR THE ENHANCEMENT OF PROTEASE PRODUCTION BY THE EFFECT OF NATURAL MICROPARTICLES

Abstract

Even though fungal proteases under interest, bulk fungal growth during the productions decreases the overall mass transfer and consequently the yield. In this study, instead of inorganic microparticles, various organic microparticles were used in the production medium to prevent bulky fungal growth and increase the homogeneity. Results showed that all microparticle additions did not only increased the maximum enzyme activity but also decreased the required time to reach the highest value. Among organic microparticle addition productions, the highest protease activity was reported as 100,76 U/ml in walnut shells added flasks, which was approximately 1,4 fold higher compared to highest activity obtained in the control production. It was also reported that microparticle addition increased the homogeneity but also resulted in higher viscosity due to hyphae type growth. Additionally, evaluation of various storage temperatures showed that produced enzyme lost only its 7% activity at 4oC at the end of 25 days.