Abstract
The activity of most of the promoters in Escherichia coli, involved in the metabolism of sugars other than glucose, is controlled by a CRP (cAMP receptor protein) or CAP (catabolite activator protein). CRP-dependent promoters are differentiated into various classes (Class I, Class II, and Class III) based on its cognate binding site’s position on DNA. The promoters regulated by CAP are differentially regulated by this transcriptional factor and it is also imperative to mention that these promoters vary greatly in respect to the binding site of CAP to its cognate binding site, it has also been reported that either it overlaps with the binding site of RNA polymerase or it present upstream to it. In Class I CAP-dependent promoters, a particular CAP molecule makes protein-protein interaction for the start of transcription. In Class II CAP-dependent promoters, a particular CAP molecule makes multiple interactions for the start of transcription. At last, in Class III-CAP dependent promoters, more than one CAP molecule is involved and activation of transcription is done synergistically. It has also been documented that CAP shows a kind of biphasic behavior in some promoters. So, the main focus of this work is to find out whether this biphasic behavior is true for other E. coli promoters as well. Experiments have been performed to know more about this biphasic nature and the various patterns of interactions of catabolite activator protein (CAP) of E. coli with its different classes of promoters.
Publisher
Journal of Experimental Biology and Agricultural Sciences
Subject
General Agricultural and Biological Sciences,General Veterinary,General Biochemistry, Genetics and Molecular Biology