<i>Csf1r+;Cd68</i>+ Macrophages Uniquely Express <i>Lactotransferrin</i> and <i>Vegfc</i> During Complex Tissue Regeneration in Spiny Mice

Author:

Simkin Jennifer,Adam Mike,Aloysius Ajoy,Safaee Fatemah,Biswas Shishir,Lakhani Zohaib,Gensel John C.,Thybert David,Potter Steven,Seifert Ashley Winn

Publisher

Elsevier BV

Subject

General Earth and Planetary Sciences,General Environmental Science

Reference144 articles.

1. Tissue embedded in paraffin and prepared 5�M section. RNAscope Multiplex fluorescent Detection Reagent V2 kit (ACD, Cat. No. 323110) were used to hybridize the species-specific probes for Cd14, Csf1r, Ltf and Pdgfa on the section as per the manufacturer's protocol. Briefly, deparaffinized tissue sections were pretreated sequentially with hydrogen peroxide (10 min, at RT), RNAscope 1X Target retrieval buffer (15min, at 100?C) and protease (30 min at 40?C);Wang;RNAscope RNA in situ hybridization was performed using RNAscope� technology,2012

2. Osteal macrophages promote in vivo intramembranous bone healing in a mouse tibial injury model;K A Alexander;J Bone Miner Res,2011

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