1. Reagents and materials Afatinib was acquired from Zhengzhou Acme Chem Co., Ltd;Ltd
2. Total GLP-1 ELISA kit and total GLP-2 ELISA kit were acquired from;The Prime Script RT Reagent Kit and SYBR Premix Ex Taq Kit were purchased from Takara Biomedical Technology
3. All animal studies were conducted following the research proposals outlined by the Committee for Research and adhering to the guidelines of the Committee on the Care and Use of Laboratory Animals in China. These protocols were approved by the Animal Ethics Committee of Central South University. Rats were acclimatized for three days in a 12 h light-dark cycle;Animals Male Sprague-Dawley (SD) rats weighing between 180-220 g were sourced from Hunan Slake Jingda Experimental Animal Co., Ltd,2019
4. Review of EGFR TKIs in Metastatic NSCLC;B Melosky;Including Ongoing Trials. Front Oncol,2014
5. BIBW2992, an irreversible EGFR/HER2 inhibitor highly effective in preclinical lung cancer models;D Li;Oncogene,2008